FastQCFastQC Report
Thu 2 Jun 2016
DRR024927_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024927_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3640816
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93790.2576070858840436No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85570.23502972959907892No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53750.14763173969791388No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61000.058.4545941
GTATCAA117000.041.6938671
ATCAACG146800.032.731013
TCAACGC148700.032.3373034
TATAACG3900.031.3431992
TATCAAC155050.031.2320142
CAACGCA155050.031.1342055
AACGCAG163950.029.53016
GTGGTAT25750.028.6814881
TGGTATC25050.027.964892
ATAACGC5400.026.9899753
CGCAGAG179950.026.560168
ACGCAGA182250.026.2769057
TCTGTCG12300.025.981578
TAACGCA5600.024.34164
GCAGAGT194950.023.7692389
GTACATG98250.022.6947331
ACAACGC6250.022.56713
CTGTCGC14050.022.410949
TACATGG94500.021.9899882
Produced by FastQC (version 0.11.5)