FastQCFastQC Report
Thu 2 Jun 2016
DRR024926_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024926_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4037212
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174300.43173358248216837No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139120.3445942397872591No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92470.22904420179074075No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94650.055.6356851
GTATCAA179600.043.195021
ATCAACG230400.033.666113
TCAACGC232900.033.303084
TATCAAC238650.032.466922
CAACGCA245750.031.5991735
AACGCAG255700.030.4779746
GTGGTAT42100.028.0315881
ACGCAGA281750.027.4598527
CGCAGAG283200.027.1532828
GTACATG197200.027.0372311
TGGTATC42800.026.6936382
TACATGG192550.026.517442
GCAGAGT298850.025.1965779
ACATGGG201500.024.5665863
CATGGGG94700.021.44394
GAGTACT192550.021.27131512-13
AGAGTAC281000.021.1098610-11
CAGAGTA293900.021.01493610-11
CATGGGA87450.020.7490144
Produced by FastQC (version 0.11.5)