FastQCFastQC Report
Thu 2 Jun 2016
DRR024926_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024926_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4037212
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192230.4761454191655033No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161000.398790056108027No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110840.2745458994969796No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44410.1100016546071893No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA95550.056.118411
GTATCAA182350.043.2830051
ATCAACG240750.032.6099363
TCAACGC243750.032.2974134
TATCAAC246450.031.8557222
GTGGTAT40450.031.628721
TGGTATC41350.031.0375142
CAACGCA254700.030.9088925
AACGCAG265000.029.7962076
ACGCAGA299700.026.140847
CGCAGAG301500.025.9536028
GTACATG188700.024.7768291
GCAGAGT316750.024.258949
TACATGG184450.024.0088882
ACATGGG196500.021.7231623
AGAGTAC301450.021.00745210-11
AGTACTT213250.020.75947812-13
GAGTACT212400.020.44428812-13
TACCTGG26800.020.3480592
CAGAGTA313250.020.11109410-11
Produced by FastQC (version 0.11.5)