FastQCFastQC Report
Thu 2 Jun 2016
DRR024925_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024925_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3360250
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107580.32015475039059593No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88770.26417677256156535No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54210.16132728219626516No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62300.056.827731
GTATCAA113900.039.9993931
TCAACGC145200.031.242764
ATCAACG144400.031.189823
CAACGCA149750.030.2306965
TATCAAC149900.030.142212
GTGGTAT26900.029.1888871
AACGCAG156650.029.1357236
TAACGCA4250.028.7589554
TACCTGG29950.027.4722712
TATAACG3750.026.3294242
TGGTATC27400.025.9107342
ACGCAGA174950.025.7115637
GTACCTG35250.025.6091671
GTACATG103500.025.5299131
CGCAGAG176950.025.340898
ATAACGC5800.025.1274413
TACATGG103250.023.7247142
GTCGTAT1401.9274048E-523.5084131
GCAGAGT187300.023.037169
Produced by FastQC (version 0.11.5)