FastQCFastQC Report
Thu 2 Jun 2016
DRR024925_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024925_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3360250
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115360.34330778959898817No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100260.2983706569451678No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67400.20058031396473475No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63250.058.2249221
GTATCAA117200.041.2948071
ATCAACG150600.031.5886083
TCAACGC151150.031.533524
TATCAAC156750.030.529182
CAACGCA157850.030.2546255
AACGCAG164200.029.2849926
GTGGTAT27350.028.2029291
TGGTATC27250.027.0837062
TACCTGG28300.026.7432632
TATAACG3300.025.6409132
ACGCAGA185500.025.591037
CGCAGAG187500.025.2679238
ACAACGC6400.024.2386743
GTACCTG32050.023.7735841
GTACATG102150.023.7124581
GCAGAGT199650.023.3064529
TACATGG100400.023.1296042
TAACGCA4100.021.7827344
ACATGGG104550.021.4920983
Produced by FastQC (version 0.11.5)