FastQCFastQC Report
Thu 2 Jun 2016
DRR024924_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024924_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3172377
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89280.2814293509251895No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74860.2359744759213675No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45070.14207012596548266No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49550.055.4097481
GTATCAA97500.043.9269261
ATCAACG123550.034.2813533
TCAACGC125550.033.658784
TATCAAC128450.033.2329372
GTGGTAT21800.032.3483241
CAACGCA131650.032.1721345
AACGCAG138150.030.8606416
TCTGTCG8000.030.5535668
TGGTATC22900.029.3573992
ACGCAGA154350.027.4084457
CGCAGAG154600.027.2729118
GCAGAGT167500.024.695429
CTGTCGC11150.023.1865629
GTACATG85800.022.7941321
TACATGG82750.022.611642
GTACCTG23500.022.0060391
AGAGTAC157550.021.33222810-11
TACCTGG24200.020.9809632
ACAACGC5900.020.715593
Produced by FastQC (version 0.11.5)