FastQCFastQC Report
Thu 2 Jun 2016
DRR024924_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024924_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3172377
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98720.3111862177792866No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86190.27168902056722766No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59590.18784022201648795No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57350.056.0951161
GTATCAA105800.043.743331
ATCAACG138700.033.1773383
TCAACGC141200.032.5540664
TATCAAC144050.032.0756572
GTGGTAT23950.031.224391
CAACGCA147250.031.1207735
ATAACGC3950.030.9393773
AACGCAG154250.029.9822446
TAACGCA4350.029.172634
TGGTATC24250.029.074672
ACGCAGA173050.026.6138447
CGCAGAG172850.026.3731698
TATAACG4450.024.2942282
GCAGAGT184100.024.1744299
GTACATG79950.023.6488481
ACAACGC5450.022.4239523
TACATGG81850.022.1668452
AGAGTAC174750.021.46045110-11
ACATGGG83350.020.922023
Produced by FastQC (version 0.11.5)