FastQCFastQC Report
Thu 2 Jun 2016
DRR024923_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024923_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5665062
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137390.24252161759218172No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113760.2008098057885333No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68780.12141085128459317No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA92850.054.534361
GTATCAA178500.042.9324651
ATCAACG222050.033.999893
TCAACGC225650.033.435154
TATCAAC235750.032.1474042
CAACGCA238650.031.694585
AACGCAG249850.030.4216466
ACGCAGA277550.027.1648647
CGCAGAG278350.026.8503728
GTACATG211600.025.5516281
GTGGTAT41050.025.196831
GCAGAGT298250.024.5229959
TACATGG206650.024.457142
TGGTATC42600.023.0658382
ACATGGG211700.023.0493773
AGAGTAC278100.020.7643610-11
TACCTGG42600.020.7482192
GAGTACT184750.020.57944712-13
CAGAGTA296550.020.03519210-11
GTACCTG47200.019.5231761
Produced by FastQC (version 0.11.5)