FastQCFastQC Report
Thu 2 Jun 2016
DRR024923_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024923_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5665062
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152590.26935274494789285No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129130.22794101812124914No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87570.15457906727234405No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA100250.054.3777281
GTATCAA189000.042.9288561
ATCAACG241050.033.2306633
TCAACGC246300.032.55624
TATCAAC253000.031.9583572
CAACGCA257200.031.2495785
AACGCAG269700.029.8704136
GTGGTAT44650.026.0194131
ACGCAGA306700.025.9731797
CGCAGAG308100.025.6568588
TGGTATC45600.024.5351452
GTACATG216400.024.5173681
TACATGG210800.023.3704592
GCAGAGT330150.023.2457779
ACATGGG216300.021.9286143
AGAGTAC308700.020.49884210-11
GAGTACT208050.020.34097512-13
AGTACTT212450.019.6984912-13
TATAACG4550.019.6299292
CAGAGTA324750.019.62321510-11
Produced by FastQC (version 0.11.5)