FastQCFastQC Report
Thu 2 Jun 2016
DRR024922_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024922_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3518804
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181940.5170506797195866No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144530.41073614785023543No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94020.2671930576411758No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39960.11356131230952334No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39530.11233930619608253No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA88450.057.512361
GTATCAA173550.043.0729141
TATAACG3250.039.057642
TACCTGG28300.036.049472
ATCAACG223400.033.351563
TCAACGC227000.032.8612484
TATCAAC231750.032.2148672
GTGGTAT35000.031.8355261
CAACGCA239200.031.244615
TGGTATC35600.030.37412
AACGCAG250550.029.9400796
GTACCTG35000.029.5519661
ACCTGGG33850.029.3017653
GTACATG203100.028.4957351
TACATGG197900.027.8424472
CGCAGAG272750.027.0895798
ACGCAGA274350.027.0001227
ACATGGG206050.025.6655123
GCAGAGT282400.025.5314319
CTATGCG1151.377639E-424.5226769
Produced by FastQC (version 0.11.5)