FastQCFastQC Report
Thu 2 Jun 2016
DRR024922_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024922_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3518804
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200670.5702789925213226No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165150.46933560380174627No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116390.3307657942869225No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44820.127372823266087No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37410.10631453186935105No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36060.10247800104808337No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA89700.056.5160941
GTATCAA172050.043.323191
GTGGTAT34100.035.8562471
TACCTGG25900.035.0277332
TGGTATC36100.033.0735632
ATCAACG225500.032.706263
TCAACGC229450.032.282954
TATCAAC234600.031.457642
CAACGCA239500.031.0264035
TATAACG4600.030.656172
AACGCAG250350.029.7568366
GTACATG197100.028.1779331
TACATGG191750.027.7011322
ACCTGGG32600.026.6752553
CGCAGAG281950.026.203228
ACGCAGA284400.026.1265857
GTACCGG3600.026.1260241
GTACCTG36750.025.7208061
ACATGGG199350.025.5368123
GCAGAGT296350.024.5176459
Produced by FastQC (version 0.11.5)