FastQCFastQC Report
Thu 2 Jun 2016
DRR024921_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024921_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2354336
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69570.29549732918326016No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58180.24711850814836964No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36270.1540561754991641No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45550.050.5773351
GTATCAA78950.037.81551
ATCAACG95350.030.864253
TCAACGC96900.030.5141494
TATCAAC97750.030.2539482
CAACGCA100100.029.6319665
AACGCAG104050.028.6413766
TACCTGG19650.028.4729612
ACAACGC3750.027.579953
TCTGTCG8300.026.6163228
ACGCAGA113550.025.9128677
GTACCTG22100.025.7419411
CGCAGAG114450.025.3805458
CTGTCGC10200.023.9619549
GTGGTAT22450.023.6652071
TATAACG2002.2922904E-823.5081372
GCAGAGT122650.023.3382769
GTACATG59500.022.8364771
GTCTCGC6150.022.1702771
TGGTATC22600.022.0518822
Produced by FastQC (version 0.11.5)