FastQCFastQC Report
Thu 2 Jun 2016
DRR024921_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024921_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2354336
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77920.3309638046565996No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69510.2952424802577033No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46470.19738049284384215No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48600.042.8785631
GTATCAA84200.039.7219281
ATCAACG105300.031.0298753
TCAACGC106500.030.7191644
TATCAAC109850.029.9158082
CAACGCA110350.029.8177935
AACGCAG114600.028.7529986
TACCTGG19800.027.0684412
ACGCAGA127100.025.6989427
CGCAGAG128350.025.2655758
GTACCTG21800.024.5992091
ACAACGC3500.024.1784253
GTACATG58800.023.6802981
GCAGAGT136300.023.4126159
TATAACG2453.3833203E-1023.0270732
TAACGCA2704.7293724E-1122.6323364
TCTGTCG7550.022.4094688
AGAGTAC121050.022.24600210-11
TACATGG60800.021.8056532
ACATGGG61250.020.8011253
Produced by FastQC (version 0.11.5)