FastQCFastQC Report
Thu 2 Jun 2016
DRR024920_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024920_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3551071
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83680.23564721741694267No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76710.2160193361383087No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43110.12139999453685946No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64050.055.491921
GTATCAA116050.040.5523451
ATCAACG143100.032.358853
TCAACGC143650.032.2640464
CAACGCA148600.031.2196165
TATCAAC149050.031.1639182
AACGCAG157000.029.6972876
GTGGTAT30850.027.1263831
ACGCAGA171700.027.0726497
CGCAGAG170850.026.959758
TGGTATC29350.026.9109022
GTACATG94050.024.8441811
GCAGAGT183400.024.3460799
TACATGG92800.023.2030242
GAGTACT130550.022.06726812-13
ACATGGG96600.021.850723
CAGAGTA183350.021.1612310-11
AGAGTAC169050.020.99116510-11
GTCTCGC9500.020.7851311
TCTGTCG10300.020.0779888
Produced by FastQC (version 0.11.5)