FastQCFastQC Report
Thu 2 Jun 2016
DRR024920_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024920_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3551071
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90500.25485269092057017No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84360.23756213266363865No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50590.1424640622505154No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66700.056.2052651
GTATCAA114200.036.6991581
ATCAACG141400.029.557693
TCAACGC142700.029.2838784
CAACGCA148600.028.184465
TATCAAC148000.028.1760432
TGGTATC28150.028.0575032
GTGGTAT29250.027.6596761
AACGCAG155150.027.085486
CGCAGAG175100.023.807568
ACGCAGA176450.023.6786827
GTACATG98100.023.3509461
TACATGG95850.022.071842
GCAGAGT188250.021.6202329
ACATGGG97250.020.690563
TATAACG3001.9281288E-1020.3723072
GAGTACT137200.019.96951112-13
CAGAGTA190550.019.59494410-11
AGAGTAC178150.019.44199210-11
TCTGTCG10250.018.7991148
Produced by FastQC (version 0.11.5)