FastQCFastQC Report
Thu 2 Jun 2016
DRR024919_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024919_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3541311
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198910.5616846416482483No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153110.43235400675060737No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106480.3006796070720702No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA48120.135881881032194No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40730.11501390304325149No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38280.10809556122012441No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA92900.059.861221
GTATCAA185400.042.5713581
TACCTGG28950.038.3207322
ATCAACG236650.033.0719453
TCAACGC240550.032.5162124
ACCTGGG33900.031.8918843
TATCAAC248600.031.4835382
TATAACG3750.031.3385682
CAACGCA251600.031.0120935
TACAACG8500.030.969882
TGGTATC34600.030.5686912
GTGGTAT35550.030.1489221
AACGCAG263350.029.8417516
GTACCTG39200.029.740081
GTACATG211350.028.2918551
TACATGG204900.027.8284662
GTACCGG2053.6379788E-1127.517181
ACGCAGA287350.026.9240237
CGCAGAG287750.026.8049268
ACATGGG216350.025.398593
Produced by FastQC (version 0.11.5)