FastQCFastQC Report
Thu 2 Jun 2016
DRR024919_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024919_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3541311
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223830.6320540613349125No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180710.5102912452478757No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135100.3814971348181507No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46690.131843828457879No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43120.12176281608703668No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA42550.12015324268328875No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA100150.056.26191
GTATCAA195500.043.280571
TATAACG4700.042.0069122
TACCTGG28150.038.574732
GTACCGG2750.034.206281
TACAACG8350.032.6520772
ACAACGC9550.032.487023
ATCAACG260750.032.3240133
ACCTGGG33100.031.811883
TCAACGC266600.031.5939714
TATCAAC274300.030.8300822
GTACCTG35900.030.7880361
GTGGTAT39750.030.6458191
TGGTATC39400.030.5430972
CAACGCA277400.030.3808695
AACGCAG290650.029.0443956
TAACGCA6300.028.3510684
GTACATG210100.027.7590241
TACATGG202800.027.0271282
ACGCAGA321150.026.0188967
Produced by FastQC (version 0.11.5)