FastQCFastQC Report
Thu 2 Jun 2016
DRR024918_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024918_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4720006
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105420.22334717371121984No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88900.18834721820268874No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53330.11298714450786716No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69200.052.7244031
GTATCAA131150.042.589661
ATCAACG161700.034.045283
TCAACGC163450.033.6775554
CAACGCA171750.032.131485
TATCAAC172050.032.0825922
AACGCAG180650.030.8330696
GTGGTAT34550.027.7611961
ACGCAGA201850.027.1522737
CGCAGAG203350.026.6512078
TGGTATC34750.026.3837092
GTACATG128350.026.2651161
TACATGG127900.024.5194842
GCAGAGT220550.024.0612759
ACATGGG129700.022.980493
TATAACG3700.021.6024632
TACCTGG34750.020.7010632
AGAGTAC205600.020.60982910-11
GAGTACT154200.019.7801712-13
CAGAGTA218350.019.75079710-11
Produced by FastQC (version 0.11.5)