FastQCFastQC Report
Thu 2 Jun 2016
DRR024918_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024918_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4720006
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118540.25114374854608235No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102690.21756328275853884No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65990.13980914431040978No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75400.051.5928081
GTATCAA139450.041.8608741
ATCAACG176550.032.2990953
TCAACGC177350.032.2284774
CAACGCA183950.031.14885
TATCAAC186650.030.8535612
AACGCAG192900.029.849796
GTGGTAT34800.028.655741
ACGCAGA219300.025.9950437
TGGTATC37550.025.9153842
CGCAGAG220650.025.580418
GTACATG130850.023.2587091
GCAGAGT240800.022.9324139
TACATGG130450.021.8746872
AGAGTAC227350.020.40240710-11
ACATGGG134500.020.3422013
TACCTGG34700.020.3216512
GAGTACT168150.019.64796812-13
AGTACTT172450.019.34881412-13
CAGAGTA237200.019.3174210-11
Produced by FastQC (version 0.11.5)