FastQCFastQC Report
Thu 2 Jun 2016
DRR024917_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024917_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3643364
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95210.26132442435068254No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83100.22808591181117233No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48620.13344809906449095No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59100.056.880921
GTATCAA111800.041.128711
TCAACGC136050.032.9966164
ATCAACG135950.032.9885753
GTGGTAT24900.032.8547331
TATCAAC141650.031.7969192
TGGTATC24850.031.7847652
CAACGCA142950.031.4039155
AACGCAG150050.030.2279156
ACGCAGA167200.027.0149387
GTACATG91950.026.9467831
CGCAGAG168050.026.766428
TACATGG90250.025.9949762
ACATGGG92500.024.2421443
GCAGAGT182500.024.1320159
TATAACG3000.023.5074842
CAGAGTA179450.020.94073310-11
GAGTACT137000.020.85701812-13
AGAGTAC170400.020.77027710-11
TCTGTCG10250.020.635058
Produced by FastQC (version 0.11.5)