FastQCFastQC Report
Thu 2 Jun 2016
DRR024917_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024917_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3643364
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107320.2945629368901927No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96830.2657708645087342No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60540.1661651155360815No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60750.056.05991
GTATCAA113300.041.6834681
ATCAACG142000.032.8095933
GTGGTAT27500.032.4998171
TCAACGC143650.032.3592834
TATCAAC147000.031.7895642
CAACGCA149350.031.0928045
TGGTATC28300.030.566582
AACGCAG156950.029.7369296
ACAACGC5750.029.434063
TATAACG4000.027.0323222
ACGCAGA180350.025.5902027
CGCAGAG181850.025.1982128
GTACCGG2501.6370905E-1124.460391
GTACATG88550.024.2234671
ATAACGC4500.024.0287283
GCAGAGT197500.022.8445459
TACATGG88450.022.7489532
TAACGCA5100.022.1181724
TACAACG7550.021.7939822
Produced by FastQC (version 0.11.5)