FastQCFastQC Report
Thu 2 Jun 2016
DRR024916_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024916_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3344424
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90840.27161627831877777No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75190.22482197233365148No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45700.13664535357957006No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56350.056.1584051
GTATCAA105950.041.407051
ATCAACG127350.033.8177453
TCAACGC128750.033.518044
GTGGTAT24500.032.626951
TATCAAC133400.032.4636542
CAACGCA135350.031.848415
TATAACG3250.031.829732
TGGTATC25300.030.665992
AACGCAG142300.030.5535416
ATAACGC3400.030.422293
TACCTGG26650.028.4067972
CGCAGAG157050.027.2051228
GTACCTG28550.027.175121
ACGCAGA158000.027.1605427
GTCTCGC9600.025.9596161
ACAACGC6400.025.712023
GCAGAGT166750.024.8615079
TCTGTCG10800.024.3718788
TACAACG6800.024.2020842
Produced by FastQC (version 0.11.5)