FastQCFastQC Report
Thu 2 Jun 2016
DRR024916_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024916_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3344424
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101230.3026829134105006No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89480.26754980827789776No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58190.17399109682265165No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57400.054.086841
GTATCAA105300.040.963831
ACAACGC4200.034.6970563
GTGGTAT25150.032.918061
ATCAACG134300.031.6426323
TCAACGC136500.031.1610224
TATAACG2900.030.7989442
TATCAAC138350.030.7842982
TGGTATC25350.030.4120792
CAACGCA143050.029.8327835
AACGCAG151450.028.3022776
TACCTGG27650.028.222352
TCTGTCG10650.026.4767688
ATAACGC3250.026.0357063
GTACCTG30800.025.9631731
CGCAGAG169700.024.8689738
ACGCAGA170500.024.7802187
GTCTCGC10700.022.8601531
CTGTCGC12550.022.8428049
GCAGAGT182450.022.7446969
Produced by FastQC (version 0.11.5)