FastQCFastQC Report
Thu 2 Jun 2016
DRR024915_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024915_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4399941
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106500.24204870019847993No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87210.19820720323295246No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55700.12659260658267918No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA81550.047.9644131
GTATCAA152900.038.649831
ATCAACG182950.031.6044373
TCAACGC184700.031.2770544
TATCAAC190550.030.4703772
CAACGCA191050.030.1893065
AACGCAG200700.028.7829596
GTACATG136000.026.3757861
ACGCAGA220500.025.8572987
CGCAGAG219050.025.8350458
TACATGG131900.025.3065742
ACATGGG137400.023.2647043
GCAGAGT237250.023.1993969
AGAGTAC208450.020.98169110-11
GAGTACT150300.020.9500512-13
AGTACTT157150.019.97704912-13
GTACAGG25450.019.3964081
CAGAGTA234600.019.3341610-11
GTACTTT163900.019.13965814-15
TATACCG3507.4578566E-1118.80255
Produced by FastQC (version 0.11.5)