FastQCFastQC Report
Thu 2 Jun 2016
DRR024915_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024915_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4399941
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121430.27598097338123395No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101100.22977580835743025No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69170.15720665345285312No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94900.046.2963181
GTATCAA164850.037.437831
ATCAACG204450.029.7997133
TCAACGC207600.029.3435424
TATCAAC213450.028.7634642
CAACGCA213700.028.6159175
AACGCAG221500.027.6294486
ATAACGC4550.025.8299053
TATAACG4200.025.7438052
GTACATG139600.024.3623221
CGCAGAG248950.024.2014688
ACGCAGA250550.024.1594647
TACATGG135450.023.774192
ACATGGG135850.022.2507933
GCAGAGT266000.022.2264339
AGAGTAC230550.021.60711110-11
GAGTACT169000.020.99513612-13
ACAACGC6750.020.8935223
TACCTGG31900.020.6315352
AGTACTT179150.020.05483612-13
Produced by FastQC (version 0.11.5)