FastQCFastQC Report
Thu 2 Jun 2016
DRR024914_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024914_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4518667
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127630.28245055455513757No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111780.2473738383465743No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72190.15975950429628916No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78250.054.3396261
GTATCAA144850.040.7853051
ATCAACG182650.031.9385663
TCAACGC183500.031.812724
TATCAAC190850.030.6894552
CAACGCA191450.030.540455
AACGCAG200950.029.0966386
GTGGTAT36450.027.0990681
TGGTATC36000.026.7679212
GTACATG148200.026.6284771
ACGCAGA226850.025.5438697
CGCAGAG228750.025.2289778
TACATGG145400.025.1523462
GCAGAGT244950.023.0807849
ACATGGG150500.023.0506523
AGAGTAC233250.020.29929510-11
TACCTGG33600.020.2857952
CAGAGTA242400.019.86263710-11
TATAACG4500.019.8474352
GAGTACT170950.019.69720812-13
Produced by FastQC (version 0.11.5)