FastQCFastQC Report
Thu 2 Jun 2016
DRR024913_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024913_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3276038
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81810.24972237806765366No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68490.209063509031336No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41960.12808154239969133No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51800.051.2812651
GTATCAA92700.040.9292561
TACCTGG28900.032.861472
ATCAACG113850.032.7023663
TCAACGC114050.032.684244
CAACGCA119200.031.3101275
TATCAAC120350.031.0957052
AACGCAG123750.030.2710296
GTGGTAT21350.026.8659381
ACGCAGA139500.026.3816327
GTACCTG38300.025.7786731
GTACATG103950.025.3281571
CGCAGAG146200.024.9471998
ACCTGGG37050.024.8688033
TACATGG102750.024.0220952
ACATGGG103250.023.1292023
GCAGAGT157350.022.6716169
TGGTATC26500.020.9348342
TATAACG3401.8189894E-1220.7417682
ATAACGC3401.8189894E-1220.7395523
Produced by FastQC (version 0.11.5)