FastQCFastQC Report
Thu 2 Jun 2016
DRR024913_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024913_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3276038
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94900.2896791795455364No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80270.24502157789378512No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55370.1690151335240922No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53950.054.9273571
GTATCAA94300.040.0038951
TACCTGG31600.030.4977362
ATCAACG121350.030.449743
TCAACGC123500.029.8394134
TATCAAC128000.029.16162
CAACGCA128800.028.6845385
AACGCAG132850.027.8454576
GTACCTG37700.026.7000481
GTGGTAT24300.025.7445321
GTACATG101050.025.3687921
TATAACG2850.024.7427082
ACCTGGG39350.024.2522543
TGGTATC25650.024.009592
ACGCAGA154100.023.6668597
TACATGG104800.023.0121352
CGCAGAG159600.022.733488
ACATGGG105950.021.9636783
GCAGAGT172500.020.5429949
TAGGTCG2151.1942338E-619.676385
Produced by FastQC (version 0.11.5)