FastQCFastQC Report
Thu 2 Jun 2016
DRR024912_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024912_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5243084
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107490.2050129275060251No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90110.17186449807021975No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82250.050.986371
GTATCAA148000.040.4382061
ATCAACG177900.032.926263
TCAACGC179500.032.5252154
TATCAAC187600.031.601482
CAACGCA187150.031.2955765
AACGCAG195250.030.0199156
ACGCAGA219750.026.330757
CGCAGAG217600.026.2231928
GTGGTAT40650.025.0971471
GTACATG143500.024.5061381
TGGTATC38850.024.0817052
TACATGG142400.023.6059382
GCAGAGT237150.023.3479069
ACATGGG143800.022.1324793
TAACGCA5750.022.0728764
GAGTACT157150.020.32128512-13
AGAGTAC217450.020.08084910-11
CAGAGTA233950.019.76959610-11
GTACCGG4850.019.3871271
Produced by FastQC (version 0.11.5)