FastQCFastQC Report
Thu 2 Jun 2016
DRR024912_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024912_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5243084
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118450.2259166551594443No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105730.20165612452518403No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66780.12736778582986655No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83650.053.030931
GTATCAA150100.039.5514261
ATCAACG186750.031.2155513
TCAACGC188450.030.9527054
CAACGCA197200.029.6028485
TATCAAC199850.029.3575922
AACGCAG204850.028.6120746
ACGCAGA235200.024.6978177
CGCAGAG234650.024.6553278
GTACATG139850.024.0843011
GTGGTAT40450.023.4918021
TACATGG140900.022.6552472
TGGTATC39850.022.5327932
GCAGAGT255600.022.0828659
TACCTGG40450.021.3849982
ACATGGG144550.021.2375833
GTACCTG41800.021.1575361
AGAGTAC234250.020.4236410-11
GAGTACT173450.019.8062312-13
CAGAGTA253800.019.10966310-11
Produced by FastQC (version 0.11.5)