FastQCFastQC Report
Thu 2 Jun 2016
DRR024911_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024911_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3853824
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92510.24004728809618706No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80310.20839041948983658No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45990.11933601534475886No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63600.056.9917641
GTATCAA119900.041.9937931
ATCAACG145300.033.6804123
TCAACGC147800.033.2039834
TATCAAC152950.032.335612
CAACGCA154250.031.8460245
AACGCAG160450.030.7880386
GTGGTAT27000.030.1229271
TGGTATC27600.027.7647232
ACGCAGA180250.027.066717
CGCAGAG180700.026.6871768
GCAGAGT196500.023.871599
GTACATG104200.022.7393281
GTACCTG30450.022.5413741
ACAACGC6750.022.2863253
TACCTGG31000.021.8381392
GAGTACT135550.021.04609712-13
AGAGTAC182950.020.79686410-11
CAGAGTA194200.020.63281810-11
TACATGG107200.020.6118952
Produced by FastQC (version 0.11.5)