FastQCFastQC Report
Thu 2 Jun 2016
DRR024911_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024911_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3853824
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106230.2756482911518533No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94290.24466607712235952No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57900.15024038461538464No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62700.058.895941
GTATCAA123850.043.452351
ATCAACG156150.034.2319073
TCAACGC158200.033.930744
TATCAAC161250.033.2952
CAACGCA164300.032.727785
AACGCAG171250.031.4270046
GTGGTAT28400.029.815181
TGGTATC27650.028.9045622
ACGCAGA195350.027.3064637
CGCAGAG193650.027.254948
GCAGAGT211150.024.5063869
AGAGTAC197500.021.1191210-11
GAGTACT149400.020.4941512-13
TACCTGG29500.020.2392462
CAGAGTA208750.020.16107410-11
GTACATG102850.019.8046341
ACAACGC6250.019.5571693
AGTACTT157250.019.3814112-13
TATAACG4200.019.0288372
Produced by FastQC (version 0.11.5)