FastQCFastQC Report
Thu 2 Jun 2016
DRR024910_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024910_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3418267
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93690.27408625481859666No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79160.23157933537666897No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47180.13802315617826225No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74650.040.372661
ATAACGC3500.034.9231643
GTATCAA122850.034.7129331
TACCTGG33500.034.2450522
GTACCTG36250.032.0367241
TCTGTCG10850.030.758638
ATCAACG144650.029.2504393
TCAACGC145100.029.2219564
CAACGCA151750.028.0351455
TATCAAC152000.027.8079472
TATAACG4250.027.6569252
AACGCAG158000.027.0423976
GTACATG86400.025.6310461
ACAACGC7050.025.3397773
GTCTCGC11100.024.567681
ACGCAGA172400.024.5382547
CGCAGAG172450.024.3948558
TACATGG87150.023.8995152
CTGTCGC14250.023.4197319
ACATGGG87550.022.8213373
Produced by FastQC (version 0.11.5)