FastQCFastQC Report
Thu 2 Jun 2016
DRR024910_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024910_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3418267
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105920.30986461853330943No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91100.26650931597795025No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61430.17971094709687685No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77850.041.5698971
TATAACG3150.040.2941252
TACCTGG32250.036.004412
GTATCAA126550.034.6791651
GTACCTG35000.034.0017781
ATAACGC4000.031.7316233
ACAACGC6150.029.0467133
TACAACG6850.027.4509852
ATCAACG159300.027.3855023
TCAACGC160400.027.1339174
TATCAAC165150.026.4723722
CAACGCA164650.026.4616895
GTACCGG3200.026.4588931
TAACGCA5000.026.320494
AACGCAG171450.025.549246
TCTGTCG10350.025.4274468
GTACATG83000.024.1424121
GTCTCGC11000.023.5190141
ACCTGGG48250.023.2856883
TACATGG83400.023.2794382
Produced by FastQC (version 0.11.5)