FastQCFastQC Report
Thu 2 Jun 2016
DRR024909_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024909_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5173153
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147720.2855511909274673No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121980.2357943018503416No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72290.13974069585801926No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA89000.054.038791
GTATCAA169500.042.7140731
ATCAACG209150.034.074033
TCAACGC211650.033.73724
TATCAAC216350.033.0512662
CAACGCA224150.031.8336075
AACGCAG235150.030.442366
ACGCAGA262500.026.947997
CGCAGAG262650.026.8249688
GTGGTAT41800.026.768291
GTACATG195400.025.2148691
GCAGAGT280600.024.2714529
TGGTATC44550.024.0604062
TACATGG199450.023.971912
ACATGGG205850.022.7452453
TACCTGG42200.021.1668832
AGAGTAC258950.020.6920710-11
CAGAGTA271500.020.45403110-11
GAGTACT173450.020.3488912-13
CATGGGG119750.019.391934
Produced by FastQC (version 0.11.5)