FastQCFastQC Report
Thu 2 Jun 2016
DRR024909_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024909_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5173153
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166840.32251124217667637No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145410.2810858290872124No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96850.18721657758817495No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA100150.053.1686331
GTATCAA183350.042.049231
ATCAACG237350.032.028543
TCAACGC242100.031.4520154
TATCAAC245900.031.106092
CAACGCA252700.030.1140965
AACGCAG265050.028.799326
GTGGTAT43900.028.2878171
TGGTATC48150.024.9954012
ACGCAGA302800.024.9734177
CGCAGAG304400.024.5951218
GTACATG211300.023.2413331
GCAGAGT320300.022.77269
TACATGG212150.022.692032
ACATGGG217450.021.2309043
TACCTGG41800.020.6946432
AGAGTAC302850.020.59247810-11
AGTACTT208300.019.87666312-13
ACAACGC10750.019.6797893
GAGTACT201650.019.42514412-13
Produced by FastQC (version 0.11.5)