FastQCFastQC Report
Thu 2 Jun 2016
DRR024908_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024908_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4394407
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158610.36093607169294967No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131860.30006323947690783No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79690.18134414950640665No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94150.055.525941
GTATCAA178450.041.3613361
ATCAACG225150.032.7166563
GTGGTAT39200.032.5008471
TCAACGC226800.032.4775354
TATCAAC232000.031.6924082
CAACGCA237450.030.8819375
TGGTATC42100.030.2617242
AACGCAG246750.029.7918246
ACGCAGA278700.026.0895127
TACCTGG36450.025.924182
CGCAGAG279500.025.8968288
GTACATG208650.025.3931711
TACATGG206900.024.4942842
GCAGAGT298150.023.7094069
ACATGGG211450.022.9426673
GTACCTG44250.022.3109191
GAGTACT181850.020.79114312-13
CATGGGG96250.020.7560774
CAGAGTA288250.020.49621210-11
Produced by FastQC (version 0.11.5)