FastQCFastQC Report
Thu 2 Jun 2016
DRR024908_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024908_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4394407
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171890.39115630391085754No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151490.3447336580339509No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100810.22940524170838067No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98150.056.161611
GTATCAA179950.041.008451
GTGGTAT39100.034.522911
ATCAACG233900.031.23173
TCAACGC236600.030.8316974
TATCAAC240950.030.3959242
TGGTATC44250.029.8516182
CAACGCA247200.029.528645
AACGCAG258800.028.223266
GTACATG207800.026.7531071
TACATGG206650.026.0007362
TATAACG4550.024.7956032
ACGCAGA297150.024.3718247
CGCAGAG295000.024.3582828
ACATGGG215100.024.1707173
TACCTGG36800.023.5041682
ATAACGC5250.023.2803153
GCAGAGT314850.022.2852389
CATGGGG98000.020.4316774
AGAGTAC298150.020.38873710-11
Produced by FastQC (version 0.11.5)