FastQCFastQC Report
Thu 2 Jun 2016
DRR024907_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024907_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3369290
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117230.34793680567716045No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96000.28492649786750324No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58470.17353804510742618No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70100.051.914921
GTATCAA122950.040.1923561
TACCTGG32400.035.2639122
GTACCTG36800.032.4530221
ATCAACG151800.032.2405973
TCAACGC153550.031.932024
TATCAAC156500.031.3056452
CAACGCA161100.030.5526925
AACGCAG167550.029.5124446
GTGGTAT30150.028.3826751
ACCTGGG40200.027.717013
GTACATG135700.026.7489471
ACGCAGA189300.025.7987587
CGCAGAG190700.025.4121768
TACATGG136350.025.311052
TGGTATC33100.025.142852
TATAACG3700.024.1446632
ACATGGG139650.023.6330783
GCAGAGT203600.023.2710869
ACAACGC8100.021.4753783
Produced by FastQC (version 0.11.5)