FastQCFastQC Report
Thu 2 Jun 2016
DRR024907_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024907_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3369290
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133150.39518711657352146No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114090.33861733480941086No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78130.23188861748320863No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69900.053.6945531
GTATCAA126500.041.7164041
TACCTGG33650.035.9053462
ATCAACG164800.031.5221753
TCAACGC167800.030.9788024
GTGGTAT30400.030.9428961
TATAACG3800.030.9291172
TATCAAC169300.030.9064622
GTACCTG40050.030.8857211
CAACGCA174250.029.9669655
AACGCAG181800.028.8000226
ACCTGGG40850.028.310933
TGGTATC33200.027.6126172
ACGCAGA207550.024.9310347
CGCAGAG208150.024.7459078
TAACGCA4850.024.2270074
GTACATG132350.024.1651521
TACATGG132800.023.3645232
ATAACGC5650.022.4660343
GCAGAGT223000.022.444719
Produced by FastQC (version 0.11.5)