FastQCFastQC Report
Thu 2 Jun 2016
DRR024906_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024906_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4341162
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107160.2468463512764555No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94890.21858202941977284No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53840.1240220936237809No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75150.049.0487251
GTATCAA133250.039.5529671
ATCAACG162650.031.9955233
TCAACGC162450.031.9458894
TATCAAC169700.030.8911232
CAACGCA169450.030.6528825
AACGCAG177600.029.3507486
ACGCAGA196450.026.1749527
CGCAGAG196300.025.8118468
GTGGTAT39650.024.7824231
GTACATG126250.024.3176981
TACATGG125850.023.685182
GCAGAGT211150.023.150639
TGGTATC40850.022.7883972
ACATGGG130000.022.0588133
GAGTACT139650.020.86501912-13
AGAGTAC193800.020.2149410-11
CAGAGTA207250.019.84422310-11
CCGATCG2151.1949596E-619.6754479
TACCTGG33450.019.5370662
Produced by FastQC (version 0.11.5)