FastQCFastQC Report
Thu 2 Jun 2016
DRR024906_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024906_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4341162
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115130.265205491064374No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105360.24269999599185657No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64060.1475641775174481No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78450.050.8458331
GTATCAA137050.039.0928341
ATCAACG174050.030.3865053
TCAACGC175050.030.2076974
TATCAAC180250.029.3934732
CAACGCA181100.029.2245035
AACGCAG190050.027.9468426
ACGCAGA215700.024.2285167
CGCAGAG216200.023.9330928
GTACATG124900.023.5003661
GTGGTAT40600.022.8240471
TACATGG125450.022.0722622
GCAGAGT233400.021.7465349
ACATGGG127750.020.5340923
TGGTATC42150.020.5221332
CCGGTCG4600.020.4333389
AGAGTAC212550.020.04316710-11
GAGTACT158200.019.66566712-13
TACCTGG32500.019.527772
ATAACGC4100.019.4924893
Produced by FastQC (version 0.11.5)