FastQCFastQC Report
Thu 2 Jun 2016
DRR024905_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024905_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6670293
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115380.17297590975388935No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96080.1440416485452738No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93250.053.3919641
GTATCAA177700.042.013791
ATCAACG212650.034.6201633
TCAACGC213750.034.4601174
TATCAAC227950.032.3396452
CAACGCA227750.032.299335
AACGCAG237350.031.1494966
ACGCAGA261850.027.8037827
CGCAGAG262200.027.5334288
GTACATG186150.025.3318351
GTGGTAT45350.024.7770391
GCAGAGT284050.024.57169
TACATGG183600.023.7631512
TGGTATC45000.022.8802282
ACATGGG189350.021.8980773
GAGTACT180250.021.11927412-13
AGAGTAC258800.020.82147810-11
CAGAGTA279450.020.41814810-11
AGTACTT193600.018.837612-13
GTACTTT200450.018.8148914-15
Produced by FastQC (version 0.11.5)