FastQCFastQC Report
Thu 2 Jun 2016
DRR024905_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024905_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6670293
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134110.2010556357869137No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110530.16570486483877095No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73130.10963536384383714No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA100200.053.697471
GTATCAA187600.041.692211
ATCAACG233350.033.260563
TCAACGC235550.033.02384
CAACGCA248900.031.3658375
TATCAAC249100.031.2519362
AACGCAG258550.030.1951546
ACGCAGA292400.026.3437717
CGCAGAG293500.026.1489628
GTGGTAT50300.024.3108921
GTACATG189950.023.6708621
TGGTATC48100.023.6516042
GCAGAGT318600.023.4693349
TACATGG188100.022.5178172
AGAGTAC293650.021.0855810-11
ACATGGG194500.020.8342533
GAGTACT202300.020.65233812-13
CAGAGTA311400.019.75540710-11
AGTACTT214550.019.24316612-13
GTACTTT222600.018.3679514-15
Produced by FastQC (version 0.11.5)