FastQCFastQC Report
Thu 2 Jun 2016
DRR024904_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024904_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2942692
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90780.30849303970650005No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70510.23961053348430622No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48300.16413542429856742No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60350.051.256251
GTATCAA112100.041.2655531
TACCTGG32000.039.3715972
GTACCTG32800.038.984621
ATCAACG138550.032.741373
TCAACGC139900.032.591774
ACCTGGG38450.031.909523
TATAACG3300.031.3405742
CAACGCA146550.031.2721635
TATCAAC147250.030.9361782
TAACGCA3950.029.7506754
AACGCAG154450.029.7329776
GTACATG124600.027.5424791
ACGCAGA171350.026.388547
CGCAGAG170900.026.2655018
TACATGG123000.026.2190652
GTGGTAT28250.026.126391
TGGTATC27950.025.7340322
ACATGGG124200.024.5261553
GCAGAGT180600.024.2822159
Produced by FastQC (version 0.11.5)