FastQCFastQC Report
Thu 2 Jun 2016
DRR024904_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024904_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2942692
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99960.3396889650700787No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80690.27420470779816575No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59650.20270554988425565No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61300.056.164411
GTATCAA113500.040.9836851
TACCTGG30750.040.0573462
GTACCTG34550.035.8029061
TATAACG3650.034.7773862
GTACCGG2900.032.4371451
ACCTGGG36350.032.075493
ACAACGC5750.031.0700363
ATCAACG150200.030.5809253
TCAACGC152600.030.1852134
TATCAAC157050.029.247092
CAACGCA159500.029.0562065
TAACGCA4200.027.9777874
AACGCAG167900.027.8264876
GTGGTAT26000.027.3158171
TGGTATC25800.026.9691962
GTACATG121950.025.3778441
TACATGG117250.025.1007982
ACGCAGA189450.024.3849877
CGCAGAG189400.024.316578
Produced by FastQC (version 0.11.5)