FastQCFastQC Report
Thu 2 Jun 2016
DRR024903_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024903_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4235801
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83500.19712918524737114No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71130.16792573588797016No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62700.052.6327931
GTATCAA115000.040.6735531
ATCAACG137450.033.446863
TCAACGC138350.033.2620774
TATCAAC145000.031.7716522
CAACGCA146550.031.3367985
AACGCAG154500.030.0274966
ACGCAGA176050.026.0311837
CGCAGAG176000.025.7982238
GTACATG121050.025.2815111
GTGGTAT29000.024.477431
TACATGG121700.023.5238532
GCAGAGT189250.023.2469149
ACATGGG125250.021.7676493
TGGTATC31000.021.6848342
GAGTACT119800.020.57644812-13
AGAGTAC172200.019.99400710-11
CAGAGTA184650.019.84230610-11
CAACGCG1951.131488E-519.2841845
AGTACTT124850.018.50191912-13
Produced by FastQC (version 0.11.5)