FastQCFastQC Report
Thu 2 Jun 2016
DRR024903_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024903_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4235801
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86030.20310208151893822No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79260.1871192721282232No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50780.1198828745731917No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66950.051.01011
GTATCAA115250.038.9383541
ATCAACG144200.030.7428933
TCAACGC147400.030.1647434
CAACGCA153450.028.9754545
TATCAAC154100.028.7068312
AACGCAG160550.027.811176
GTGGTAT32400.024.6816431
ACGCAGA187050.023.6929427
CGCAGAG188050.023.2920428
GTACATG116150.022.8417851
GCAGAGT200900.021.2875869
TACATGG118750.021.2192212
TGGTATC35050.021.1917762
ACATGGG118750.020.427463
AGAGTAC189000.019.53188910-11
GAGTACT136350.019.21529212-13
AGTACTT142250.018.68261312-13
CAGAGTA198800.018.53358810-11
TATAACG4350.018.3720632
Produced by FastQC (version 0.11.5)