FastQCFastQC Report
Thu 2 Jun 2016
DRR024902_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024902_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4412756
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88100.1996484736522935No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77160.17485671086278054No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44610.10109328501281284No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74050.052.504971
GTATCAA130600.039.2736741
ATCAACG161350.031.233893
TCAACGC163550.030.7534714
TATCAAC168400.029.9555722
CAACGCA171550.029.373465
AACGCAG180000.028.2015346
GTACATG148850.025.2042641
ACGCAGA200650.025.0177757
CGCAGAG198600.024.9677838
GTGGTAT36150.024.7095741
TACATGG149050.023.8772262
TGGTATC35100.023.3056982
GCAGAGT213750.022.604449
ACATGGG153500.022.5101953
GAGTACT133650.020.1487512-13
AGAGTAC198050.019.97036610-11
TACAACG8300.019.824852
CAGAGTA211750.019.61055810-11
TACCTGG32100.019.3325372
Produced by FastQC (version 0.11.5)