FastQCFastQC Report
Thu 2 Jun 2016
DRR024902_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR024902_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4412756
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101000.2288819050951378No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87850.1990819342832461No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57300.1298508233856574No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77950.053.756891
GTATCAA134500.038.9175451
ATCAACG170900.030.3667073
TCAACGC172150.030.088534
TATCAAC179250.029.2143822
CAACGCA179500.028.9867635
GTGGTAT36550.027.9218961
AACGCAG189500.027.6307416
TGGTATC37000.025.6637652
GTACATG146900.024.6513921
ACGCAGA216850.023.926387
CGCAGAG216900.023.7258578
TACATGG147350.023.6395242
ACATGGG152050.022.1977393
GCAGAGT236700.021.2051019
AGAGTAC216450.020.14873910-11
GAGTACT149000.019.6340812-13
TACCTGG35100.019.5531352
CAGAGTA230550.019.30377810-11
CATGGGG83050.018.5635324
Produced by FastQC (version 0.11.5)